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Production of recombinant bleaching enzymes from thermophilic microorganisms in fungal hosts

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Abstract

Cost-effective production of enzymes for industrial processes makes the appropriate selection of the host-vector expression system critical. We have developed two systems for the bulk production of bleaching enzymes from thermophiles. Kluyveromyces lactis has been developed as a secretion host employing expression vectors based on the 2μ-like plasmid pKD1 of Kluyveromyces drosophilarium. Our second system involves the filamentous fungus Trichoderma reesei. Fusion and nonfusion vectors have been constructed using the strong cellobiohydrolase 1 (cbh1) promoter. The KEX2 protease cleavage site and a 6 × HIS-tag have been incorporated to facilitate both cleavage and purification of the mature foreign proteins.

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Authors and Affiliations

  1. Department of Biological Sciences, Macquarie University, 2109, Sydney, NSW, Australia

    Peter L. Bergquist, V. S. Junior Te’o, Moreland D. Gibbs & K. M. Helena Nevalainen

  2. Division of Molecular Medicine, University of Auckland Medical School, 1000, Auckland, New Zealand

    Peter L. Bergquist

  3. Institute of Biochemical Technology and Microbiology, Section of Microbial Biochemistry, Technical University of Vienna, A-1040, Vienna, Austria

    Angela C. E. Cziferszky

  4. Department de Biotecnologia, FAENQUIL, CP116, Lorena-SP, Brazil

    Fabricia P. De Faria

  5. Department de Biologia Celular, IB UnB 70.910-900, Brasilia-DF, Brazil

    Maristela O. Azevedo

Authors
  1. Peter L. Bergquist
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  2. V. S. Junior Te’o
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  3. Moreland D. Gibbs
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  4. Angela C. E. Cziferszky
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  5. Fabricia P. De Faria
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  6. Maristela O. Azevedo
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  7. K. M. Helena Nevalainen
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Correspondence to Peter L. Bergquist.

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Bergquist, P.L., Te’o, V.S.J., Gibbs, M.D. et al. Production of recombinant bleaching enzymes from thermophilic microorganisms in fungal hosts. Appl Biochem Biotechnol 98, 165–176 (2002). https://doi.org/10.1385/ABAB:98-100:1-9:165

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  • DOI: https://doi.org/10.1385/ABAB:98-100:1-9:165

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