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Production of biosurfactant from a new and promising strain of Pseudomonas aeruginosa PA1

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Abstract

The Pseudomonas aeruginosa PA1 strain, isolated from the water of oil production in Sergipe, Northeast Brazil, wasevaluated as a potential rhamnolipid type of biosurfactant producer. The production of biosurfactants was investigated using different carbon sources (n-hexadecane, paraffin oil, glycerol, and babassu oil) and inoculum concentrations (0.0016–0.008 g/L) The best results were obtained with glycerol as the substrate and an initial cell concentration of 0.004 g/L. AC:N ratio of 22.8 led to the greatest production of rhamnolipids (1700 mg/L) and efficiency (1.18 g of rhamnolipid/g of dry wt).

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Author notes

  1. L. M. Santa Anna

    Present address: Centro de Pesquisas e Desenvolvimento, Leopoldo A. Miguez de Mello, CENPES-PETROBRAS, Cidade Universitária, 1 Ilha do Fundão, Av. Hum, Quadra 7, CEP 21949-900, Rio de Janeiro, Brazil

Authors and Affiliations

  1. Centro de Pesquisas da Petrobras (Petrobras Research Center-CENPES), Rio de Janeiro, Brazil

    L. M. Santa Anna & G. V. Sebastian

  2. Esco la de Química, UFR, Rio de Janeiro, Brazil

    N. Pereira Jr.

  3. PEQ/COPPE/UFRJ, Rio de Janeiro, Brazil

    T. L. M. Alves

  4. Fundacão Tropical André Tosello, Campinas, São Paulo, Brazil

    E. P. Menezes

  5. Faculdade de Farmácia, UFRJ, Rio de Janeiro, Brazil

    D. M. G. Freire

Authors
  1. L. M. Santa Anna
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  2. G. V. Sebastian
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  3. N. Pereira Jr.
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  4. T. L. M. Alves
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  5. E. P. Menezes
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  6. D. M. G. Freire
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Santa Anna, L.M., Sebastian, G.V., Pereira, N. et al. Production of biosurfactant from a new and promising strain of Pseudomonas aeruginosa PA1. Appl Biochem Biotechnol 91, 459–467 (2001). https://doi.org/10.1385/ABAB:91-93:1-9:459

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  • DOI: https://doi.org/10.1385/ABAB:91-93:1-9:459

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