Abstract
Genetic characterization and enhancement of polyhydroxybutyrate (PHB) accumulation in cyanobacteria were investigated for efficient PHB production from CO2. The genome DNAs in the PHB-accumulating strains Synechococcus sp. MA19 and Spirulina platensis NIES46 retained the highly homologous region to phaC of Synechocystis PCC6803, whereas low homology was detected in the nonaccumulating strains Synechococcus sp. PCC7942 and Anabaenacylindrica NIES19. Synechococcus sp. MA19, which accumulates PHB up to 30% of dry cell weight from CO2 as the sole carbon source, was mutated by insertion of transposon Tn5 to enhance the PHB accumulation. Genetic and physiological analysis of the mutant indicated that decreased phosphotransacetylase activity could trigger an increase of acetyl coenzyme A leading to enhancement of PHB accumulation. PHB synthase in Synechococcus sp. MA19 was probably attached to thylakoid membrane since PHB granules were associated with pigments. A genetically engineered cyanobacteria retaining soluble PHB synthase from Ralstonia eutropha accumulated pigment-free PHB granules, which is an advantage for the purification of PHB.
Similar content being viewed by others
References
Stal, L. J. (1992), FEMS Microbiol. Rev. 103, 169–180.
Campbell, J., Stevens, S. E. J., and Balkwill, D. L. (1982), J. Bacteriol. 149, 361–363.
Vincenzini, M., Sili, C., De Philippis, R., Ena, A., and Materassi, R. (1990), J. Bacteriol. 172, 2791, 2792.
Miyake, M., Erata, M., and Asada, Y. (1996), J. Ferment. Bioeng. 82, 516–518.
Suzuki, T., Miyake, M., Tokiwa, Y., Saegusa, H., Saito, T., and Asada, Y. (1996), Biotechnol. Lett. 18, 1047–1050.
Takahashi, H., Miyake, M., Tokiwa, Y., and Asada, Y. (1998), Biotechnol. Lett. 20, 183–186.
Steinbüchel, A., and Schlegel, H. G. (1991), Mol. Microbiol. 5, 535–542.
Hein, S., Tran, H., and Steinbüechel, A. (1998), Arch. Microbiol. 170, 162–170.
Liebergesell, M., and Steinbüchel, A. (1992), Eur. J. Biochem. 209, 135–150.
Liebergesell, M., and Steinbüchel, A. (1993), Appl. Microbiol. Biotechnol. 38, 493–501.
Liebergesell, M., Sonomoto, K., Madkour, M., Mayer, F., and Steinbüchel, A. (1994), Eur. J. Biochem. 226, 71–80.
Miyake, M., Kataoka, K., Shirai, M., and Asada, Y. (1997), J. Bacteriol. 179, 5009–5013.
Miyake, M. Khatipov, E., Kataoka, K., Shirai, M., Kurane, R., and Asada, Y. (1999), Photosynthesis: Mechanisms and Effects, Garab, G., ed., V, 4155–4158.
Steinbuechel, A., Aerts, K., Babel, W., Follner, C., Liebergessel, M., Hussein, M., Mayer, F., Pieper-Fuerst, U., Pries, A., Valentin, H. E., and Wieczorek, R. (1995), Can. J. Microbiol. 41, 94–105.
Allen, M. M. (1968), J. Physol. 4, 1–4.
Asada, Y., and Kawamura, S. (1986), J. Ferment. Technol. 64, 553–556.
Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989), Molecular Cloning—A Laboratory Manual, 2nd ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
Simon, R., Priefer, U., and Puhler, A. (1984), Bio/Technology. 1, 784–791.
Elhai, J., and Wolk, C. P. (1988), Methods. Enzymol,. 167, 747–754.
Dower, W. J., Miller, J. F., and Ragsdale, C. W. (1988), Nucleic Acids Res. 16, 6123–6145.
Muhlenhoff, U., and Chauvat, F. (1996), Mol. Gen. Genetics. 252, 93–100.
Pedros-Alio, C., Mas, J., and Guerrero, R. (1985), Arch. Microbiol. 143, 178–184.
Ostle, A. G. and Holt, J. G. (1982), Appl. Environ. Microbiol. 44, 238–241.
Tandeau de Marsac, N. and Houmard, J. (1988), Methods. Enzymol. 167, 318–328.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Miyake, M., Takase, K., Narato, M. et al. Polyhydroxybutyrate production from carbon dioxide by cyanobacteria. Appl Biochem Biotechnol 84, 991–1002 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:991
Issue Date:
DOI: https://doi.org/10.1385/ABAB:84-86:1-9:991