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Cellulase production of Trichoderma reesei rut C 30 using steam-pretreated spruce

Hydrolytic potential of cellulases on different substrates

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Abstract

Various techniques are available for the conversion of lignocellulosics to fuel ethanol. During the last decade processes based on enzymatic hydrolysis of cellulose have been investigated more extensively, showing good yield on both hardwood and softwood. The cellulase production of a filamentous fungi, Trichoderma reesei Rut C30, was examined on carbon sources obtained after steam pretreatment of spruce. These materials were washed fibrous steam-pretreated spruce (SPS), and hem icellulose hydrolysate. The hemicellulose hydrolysate contained, besides water-soluble carbohydrates, lignin and sugar degradation products, which were formed during the pretreatment and proved to be inhibitory to microorganisms. Experiments were performed in a 4-L laboratory fermentor. The hydrolytic capacity of the produced enzyme solutions was compared with two commercially available enzyme preparations, Celluclast and logen Cellulase, on SPS, washed SPS, and Solka Floc cellulose powder. There was no significant difference among the different enzymes produced by T. reesei Rut C30. However, the conversion of cellulose using these enzymes was higher than that obtained with logen or Celluclast cellulases using steam-pretreated spruce as substrate.

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Authors and Affiliations

  1. Department of Chemical Engineering I, Lund University, Getingevagen 60, P.O. Box 124, S-221 00, Lund, Sweden

    Zsolt Szengyel & Guido Zacchi

  2. Department of Agricultural Chemical Technology, Technical University of Budapest, Szent Gellért tér 4, H-1521, Budapest, Hungary

    Amaranta Varga & Kati Réczey

Authors
  1. Zsolt Szengyel
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  2. Guido Zacchi
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  3. Amaranta Varga
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  4. Kati Réczey
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Correspondence to Zsolt Szengyel.

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Szengyel, Z., Zacchi, G., Varga, A. et al. Cellulase production of Trichoderma reesei rut C 30 using steam-pretreated spruce. Appl Biochem Biotechnol 84, 679–691 (2000). https://doi.org/10.1385/ABAB:84-86:1-9:679

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  • DOI: https://doi.org/10.1385/ABAB:84-86:1-9:679

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