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Optimization of acid hydrolysis of sugarcane bagasse and investigations on its fermentability for the production of xylitol by Candida guilliermondii

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Abstract

The dilute-acid hydrolysis of sugarcane bagasse was optimized using a statistical experimental design resulting in hydrolysates containing 57.25 g/L of xylose, which were fermented with a high inoculum concentration (10 g/L of the yeast Candida guilliermondii IM/UFRJ 50088). The addition of urea reduced the time of conversion (t C) to 75 h (without nitrogen source addition t C>127 h), and, consequently, improving the rates of xylitol bioproduction. Fermentator experiments, using the optimized conditions, resulted in enhanced conversion rates, reducing t C to 30 h. The stability of the yeast in the hydrolysate was also verified in a 480-h cultivation.

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Authors and Affiliations

  1. Departmento de Engenharia Bioquímica, Escola de Química/CT, Universidade Federal do Rio de Janeiro, CEP: 21949-900, RJ, Brazil

    Rafael Fogel, Rafaela Rodrigues Garcia, Rebeca da Silva Oliveira, Denise Neves Menchero Palacio, Luciana da Silva Madeira & Nei Pereira Jr.

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  1. Rafael Fogel
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  2. Rafaela Rodrigues Garcia
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  3. Rebeca da Silva Oliveira
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  4. Denise Neves Menchero Palacio
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  5. Luciana da Silva Madeira
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  6. Nei Pereira Jr.
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Correspondence to Nei Pereira Jr..

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Fogel, R., Garcia, R.R., Oliveira, R.d.S. et al. Optimization of acid hydrolysis of sugarcane bagasse and investigations on its fermentability for the production of xylitol by Candida guilliermondii . Appl Biochem Biotechnol 122, 741–752 (2005). https://doi.org/10.1385/ABAB:122:1-3:0741

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  • DOI: https://doi.org/10.1385/ABAB:122:1-3:0741

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