B Cell Protocols

1. Front Matter

   Pages i-xi

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2. Characterization of B Lymphopoiesis in Mouse Bone Marrow and Spleen

   • Richard R. Hardy, Susan A. Shinton

   Pages 1-24

3. Characterization of B-Cell Maturation in the Peripheral Immune System

   • Rita Carsetti

   Pages 25-36

4. Identification of B-Cell Subsets

   • James W. Tung, David R. Parks, Wayne A. Moore, Leonard A. Herzenberg, Leonore A. Herzenberg

   Pages 37-58

5. Analysis of B-Cell Life-Span and Homeostasis

   • Irmgard Föster

   Pages 59-66

6. Differentiation of B Lymphocytes From Hematopoietic Stem Cells

   • Paulo Vieira, Ana Cumano

   Pages 67-76

7. Analysis of Lymphocyte Development and Function Using the RAG-Deficient Blastocyst Complementation System

   • Faith M. Young, Carl A. Pinkert, Andrea Bottaro

   Pages 77-90

8. Conditional Gene Mutagenesis in B-Lineage Cells

   • Stefano Casola

   Pages 91-109

9. Analysis of the Germinal Center Reaction and In Vivo Long-Lived Plasma Cells

   • Kai-Michael Toellner, Mahmood Khan, Daniel Man-Yuen Sze

   Pages 111-125

10. T-Cell-Dependent Immune Responses, Germinal Center Development, and the Analysis of V-Gene Sequences

    • Claudia Berek

    Pages 127-138

11. Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors

    • Kuo-I Lin, Kathryn Calame

    Pages 139-148

12. Immunoglobulin Class Switching

    • Sven Kracker, Andreas Radbruch

    Pages 149-159

13. In Vitro and In Vivo Assays of B-Lymphocyte Migration

    • Chantal Moratz, John H. Kehrl

    Pages 161-171

14. Analysis of Antigen-Specific B-Cell Memory Directly Ex Vivo

    • Louise J. McHeyzer-Williams, Michael G. McHeyzer-Williams

    Pages 173-188

15. B-Cell Signal Transduction

    • Tilman Brummer, Winfried Elis, Michael Reth, Michael Huber

    Pages 189-212

16. Isolation of Lipid Rafts From B Lymphocytes

    • Anu Cherukuri, Shiang-Jong Tzeng, Arun Gidwani, Hae Won Sohn, Pavel Tolar, Michelle D. Snyder et al.

    Pages 213-224

17. Molecular Single-Cell PCR Analysis of Rearranged Immunoglobulin Genes As a Tool to Determine the Clonal Composition of Normal and Malignant Human B Cells

    • Ralf Küppers

    Pages 225-238

18. Analysis of B-Cell Immune Tolerance Induction Using Transgenic Mice

    • Helen Ferry, Richard J. Cornall

    Pages 239-260

19. Analysis of B-Cell Signaling Using DT40 B-Cell Line

    • Tomoharu Yasuda, Tadashi Yamamoto

    Pages 261-270

20. B-Cell Development and Pre-B-1 Cell Plasticity In Vitro

    • Antonius G. Rolink

    Pages 271-281

B-lymphocyte development and function remains an exciting area of research for those interested in the physiology and pathology of the immune system in higher animals. While recent advances in genetics and cellular and molecular biology have provided a large spectrum of powerful new experimental tools in this field, it is both time consuming and often very difficult for a student or just any bench-side worker to identify a reliable experimental protocol in the ocean of the literature. The aim of B Cell Protocols is to provide a collection of diverse protocols ranging from the latest inventions and applications to some classic, but still frequently used methods in B-cell biology. The authors of the various chapters are all highly qualified scientists who are either the inventors or expert users of these methods. Their extensive experience in mastering a particular method provides not only the step-by-step details of a reproducible protocol, but also useful troubleshooting tips that readers will appreciate in their daily work. We hope that this book will be helpful for both beginning and experienced researchers in the field in designing or modifying an experimental approach, and exploring a biological question from multiple angles.

• Department of Microbiology, Columbia University College of Physicians and Surgeons, New York

  Hua Gu

• The Center for Blood Research, The Harvard Medical School, Boston

  Klaus Rajewsky

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