Haemophilus influenzae Protocols

1. Front Matter

   Pages i-xi

   PDF

2. The Pathogenesis of Disease Due to Nontypeable Haemophilus influenzae

   • Gail G. Hardy, Simone M. Tudor, Joseph W. St. Geme III

   Pages 1-28

3. The Pathogenesis of Disease Due to Type b Haemophilus influenzae

   • Ruth Aubrey, Christoph Tang

   Pages 29-50

4. General Methods for Culturing Haemophilus influenzae

   • Grant Poje, Rosemary J. Redfield

   Pages 51-56

5. Transformation of Haemophilus influenzae

   • Grant Poje, Rosemary J. Redfield

   Pages 57-70

6. Diagnosis of Infection

   • Alex van Belkum, Loek van Alphen

   Pages 71-92

7. Characterization of Plasmids

   • Ioanna D. Dimopoulou, Mohd-Zain Zaini, Derrick W. Crook

   Pages 93-99

8. Protein Characterization by Two-Dimensional Gel Electrophoresis

   • Phillip Cash, J. Simon Kroll

   Pages 101-118

9. Monitoring Gene Expression Using DNA Arrays

   • Tahir R. Ali, Ming-Shi Li, Paul R. Langford

   Pages 119-134

10. Gene Expression Technology

    • Xavier de Bolle, Christopher D. Bayliss

    Pages 135-146

11. The Genome Sequence of Haemophilus influenzae

    • Derek W. Hood

    Pages 147-160

12. Structural Profiling of Short-Chain Lipopolysaccharides from Haemophilus influenzae

    • Elke K. H. Schweda, James C. Richards

    Pages 161-183

13. Mutagenesis of H. influenzae

    • Mark A. Herbert

    Pages 185-209

14. Transposon Tn 10

    • Stefan Schlör, Gabriele Kemmer, Joachim Reidl

    Pages 211-224

15. In Vivo Expression of Bacterial Genes During Human Infections

    • Martin Handfield, Trevor Seifert, Jeffrey D. Hillman

    Pages 225-242

16. ELISA

    • Joyce S. Plested, Philip A. Coull, Margaret Anne J. Gidney

    Pages 243-261

17. Opsonophagocytosis Assay Using Flow-Cytometry

    • Joyce S. Plested, Philip A. Coull

    Pages 263-275

18. In Vitro Models of Infection I—Human Respiratory Tissue Organ Culture

    • Andrew M. Middleton, Paula Keig, Robert Wilson

    Pages 277-295

19. In Vitro Models of Infection II—Human Umbilical Vein Endothelial Cells (HUVECs) System

    • Mumtaz Virji, Darryl J. Hill

    Pages 297-314

20. Animal Models

    • Mumtaz Virji, Darryl J. Hill

    Pages 315-322

As the second leading bacterial cause of community-acquired pneumonia, H. influenzae accounts for many thousands of deaths per year worldwide, and is a prime candidate for the identification of targets that can lead to the development of novel vaccines and antibiotics. In Haemophilus influenzae Protocols, leading research scientists and infectious disease specialists detail in a readily reproducible format the major molecular and immunological techniques for exploring the pathogenicity of this significant bacterium. Described with step-by-step instructions to ensure robust and successful experimental results, the techniques cover plasmid analysis, proteomics, genomics, DNA array technology, gene expression, mutagenesis (transposon and nontransposon), and structural analysis. The antibody techniques presented include IVIAT, ELISA, and opsonophagocytosis assays. Also included are reviews of the pathogenesis of NTHi and Hib, methods for working with the organism, culture and storage conditions, molecular diagnosis, and systems for exploring pathogenesis in both in vitro and animal models.
Comprehensive and highly practical, Haemophilus influenzae Protocols offers a unique collection of powerful molecular methods that illuminate not only how the bacterium causes disease, but also how best to develop much-needed new vaccines and antibiotics against it.

• antibiotics
• bacteria
• infection
• infections
• infectious
• infectious disease
• tissue
• infectious diseases

"This excellent book is recommended for investigators involved in laboratory research on H. influenzae from the beginning student to the experienced investigator. The book contains abundant, detailed protocols and practical tips that will be useful for all investigators." - Clinical Infectious Diseases

"...an excellent source book and an inspiring reading." - International Journal of Medical Microbiology

"In addition to detailed information provided in the individual chapters, the book is also valuable by providing an extensive list of 841 references....there are not many microbiological books of such high quality. The authors present highly specialized information in a comprehensible and friendly way." -Folia Microbioligica

• Department of Pediatrics, John Radcliffe Hospital, Oxford, UK

  Mark A. Herbert, Derek W. Hood, E. Richard Moxon

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