Skip to main content

Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis

  • Protocol
  • 1907 Accesses

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 325))

Abstract

For developmental competence of mammalian embryos, dynamic epigenetic changes to both the maternally and paternally derived contributions to the genome of the zygote should be brought about in the early cleavage stages. DNA methylation is a typical epigenetic mark modified during pre-implantation development. Here, we describe how to analyze DNA methylation profiles in early-stage embryos.

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.00
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   109.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Kang, Y. K., Koo, D. B., Park, J. S., Choi, Y. H., Chung, A. S., Lee, K. K., et al (2001) Aberrant methylation of donor genome in cloned bovine embryos. Nat. Genet. 28, 173–177.

    Article  CAS  PubMed  Google Scholar 

  2. Millar, D. S., Warnecke, P. M., Melki, J. R., and Clark, S. J. (2002) Methylation sequencing from limiting DNA: embryonic, fixed, and microdissected cells. Methods 27, 108–113.

    Article  CAS  PubMed  Google Scholar 

  3. Santos, F., Hendrich, B., Reik, W., and Dean, W. (2002) Dynamic reprogramming of DNA methylation in the early mouse embryo. Dev. Biol. 241, 172–182.

    Article  CAS  PubMed  Google Scholar 

  4. Kang, Y. K., Koo, D. B., Park, J. S., Choi, Y. H., Kim, H. N., Chang, W. K., et al. (2001) Typical demethylation events in cloned pig embryos. Clues on speciesspecific differences in epigenetic reprogramming of a cloned donor genome. J. Biol. Chem. 276, 39,980–39,984.

    Article  CAS  PubMed  Google Scholar 

  5. Oswald, J., Engemann, S., Lane, N., Mayer, W., Olek, A., Fundele, R., et al. (2000) Active demethylation of the paternal genome in the mouse zygote. Curr. Biol. 10, 475–478.

    Article  CAS  PubMed  Google Scholar 

  6. Frommer, M., McDonald, L. E., Millar, D. S., Collis, C. M., Watt, F., Grigg, G. W., et al. (1992) A genomic sequencing protocol that yields a positive display of 5-methylcytosine residues in individual DNA strands. Proc. Natl. Acad. Sci. USA 89, 1827–1831.

    Article  CAS  PubMed  Google Scholar 

  7. Raizis, A. M., Schmitt, F., and Jost, J. (1995) A bisulfite method of 5-methylcytosine mapping that minimizes template degradation. Anal. Biochem. 226, 161–166.

    Article  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2006 Humana Press Inc.

About this protocol

Cite this protocol

Han, YM., Kim, SH., Kang, YK. (2006). Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis. In: Pells, S. (eds) Nuclear Reprogramming. Methods in Molecular Biology™, vol 325. Humana Press. https://doi.org/10.1385/1-59745-005-7:251

Download citation

  • DOI: https://doi.org/10.1385/1-59745-005-7:251

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-379-4

  • Online ISBN: 978-1-59745-005-8

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics