Abstract
Protocols for the proteome analysis of Arabidopsis tissues based on 2 D gel electrophoresis are given for leaves and roots as well as for seeds. The protocols contain a detailed description of the sample preparation step and of the solubilization of proteins. We then describe our protocols for the isoelectric focusing, the transfer of the strips from the first to the second dimension, SDS-PAGE as the second dimension of electrophoretic separation, staining of the gels using colloidal Coomassie blue, and finally drying of the gels between cellophane sheets. Notes contain additional background information on critical steps. Our protocols allow the analysis of leaves, roots, or seeds from different Arabidopsis ecotypes, from mutants or from plants subjected to various environmental conditions.
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Schlesier, B., Mock, HP. (2006). Protein Isolation and Second-Dimension Electrophoretic Separation. In: Salinas, J., Sanchez-Serrano, J.J. (eds) Arabidopsis Protocols. Methods in Molecular Biology™, vol 323. Humana Press. https://doi.org/10.1385/1-59745-003-0:381
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DOI: https://doi.org/10.1385/1-59745-003-0:381
Publisher Name: Humana Press
Print ISBN: 978-1-58829-395-4
Online ISBN: 978-1-59745-003-4
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