Abstract
We have developed methods for nanostructure fabrication relying on the size and shape of a polynucleotide to dictate the overall structure of an assemblage of individual semiconductor nanoparticles. Use of the circular plasmids pUCLeu4 and φχ 174 when anchored to a suitably derivatized substrate yields arrays of semiconductor nanoparticles matching the shapes of the biopolymer stabilizer. The viability of the methodology was confirmed using high-resolution transmission electron microscopy and selected-area electron diffraction.
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References
Alivasatos, A. P., Johnsson, K., Peng, X., Wilson, T., Loweth, C., Bruchez, M., and Schultz, P. (1996) Organization of nanocrystal molecules using DNA. Nature 382, 609–611.
Mirkin, C., Letsinger, R., Mucic, R., and Storhoff, J. (1996) A DNA-based method for rationally assembling nanoparticles into macroscopic materials. Nature 382, 607–609.
Coffer, J., Bigham, S., Li, X., Rho, Y., Pinizzotto, R., Pirtle, R., and Pirtle, I. (1996) Dictation of the shape of mesoscale semiconductor nanoparticle assemblies by plasmid DNA. Appl. Phys. Lett. 69, 3851–3853.
Mann, S. (1993) Molecular tectonics in biomineralization and biomimetic materials chemistry. Nature 365, 499–505.
Rho, Y. G. (1996) PhD dissertation, University of North Texas, Denton, TX.
Winfree, E. (1996) in DNA Computers, Proceedings of a DIMACS Workshop, (Lupton, R. and Baum, E., eds.), American Mathematical Society, pp. 199–219.
Coffer, J. L. (1997) Approaches for generating mesoscale patterns of semiconductor nanoclusters. J. Cluster Sci. 8, 159–178.
Coffer, J. L. and Chandler, R. R. (1991) Nucleotides as structural templates for the self-assembly of quantum-confined CdS clusters. Mater. Res. Soc. Symp. Proc. 206, 527–531.
Coffer, J. L., Bigham, S. R., Pinizzotto, R. F., and Yang, H. (1992) Characterization of quantum-confined CdS nanocrystallites stabilized by deoxyribonucleic acid. Nanotechnology 3, 69–76.
Bigham, S. R. and Coffer, J. L. (1995) The influence of adenine content on the properties of Q-CdS clusters stabilized by polynucleotides. Colloids Surfaces 95, 211–219.
Chandler, R. R., Bigham, S. R., and Coffer, J. L. (1993) Preparation and spectroscopic analysis of semiconductor colloids. J. Chem. Ed. 70, A7–A10.
Seeman, N. C. (2003) DNA in a material world. Nature 421, 427–431.
Chang, Y. N., Pirtle, I. L., and Pirtle, R. M. (1986) Nucleotide sequence and transcription of a human tRNA gene cluster with four genes. Gene 48, 165–174.
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Coffer, J.L., Pinizzotto, R.F., Rho, Y.G. (2005). Nanostructured DNA Templates. In: Rosenthal, S.J., Wright, D.W. (eds) NanoBiotechnology Protocols. Methods in Molecular Biology™, vol 303. Humana Press. https://doi.org/10.1385/1-59259-901-X:167
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DOI: https://doi.org/10.1385/1-59259-901-X:167
Publisher Name: Humana Press
Print ISBN: 978-1-58829-276-6
Online ISBN: 978-1-59259-901-1
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