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Transgenic Plants: Methods and Protocols pp 203–213Cite as

Histochemical and Fluorometric Assays for uidA (GUS) Gene Detection

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Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 286))

Summary

Transgenic plant production has been intimately connected to the β-glucuronidase (uidA or GUS) gene used as a reporter marker gene. The enzyme stability and the high sensitivity and amenability of the GUS assay to qualitative (histochemical assay) and to quantitative (fluorometric or spectrophotometric assay) detection are some of the reasons that explain the extensive use of uidA gene in plant genetic transformation. Methods for uidA gene detection have been thoroughly described in the literature. The aim of this chapter is to describe the basic protocols needed for GUS detection in a plant genetic transformation laboratory.

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References

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Author information

Authors and Affiliations

  1. Department of Plant Protection and Biotechnology, Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain

    Magdalena Cervera

Authors
  1. Magdalena Cervera
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Editors and Affiliations

  1. Instituto Valenciano de Investigaciones Agrarias, Valencia, Spain

    Leandro Peña

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© 2005 Humana Press Inc.

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Cite this protocol

Cervera, M. (2005). Histochemical and Fluorometric Assays for uidA (GUS) Gene Detection. In: Peña, L. (eds) Transgenic Plants: Methods and Protocols. Methods in Molecular Biology™, vol 286. Humana Press. https://doi.org/10.1385/1-59259-827-7:203

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  • DOI: https://doi.org/10.1385/1-59259-827-7:203

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-263-6

  • Online ISBN: 978-1-59259-827-4

  • eBook Packages: Springer Protocols

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