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Signal Transduction Protocols pp 111–127Cite as

Studies of Calmodulin-Dependent Regulation

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 284))

Abstract

Methods are presented for purifying bovine testes calmodulin and the calmodulin-regulated plasma-membrane calcium ATPase from human erythrocytes by calcium dependent affinity chromatography. The assay of CaM Kinase II using a synthetic peptide substrate is also described.

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Author information

Authors and Affiliations

  1. Department of Medical Pharmacology and Toxicology, Texas A&M University System Health Science Center, College Station, TX

    Paul C. Brandt

  2. Department of Molecular and Cellular Biochemistry, University of Kentucky College of Medicine, Lexington, KY

    Thomas C. Vanaman

Authors
  1. Paul C. Brandt
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  2. Thomas C. Vanaman
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Editor information

Editors and Affiliations

  1. Department of Molecular and Cellular Biochemistry, College of Medicine, University of Kentucky, Lexington, KY

    Robert C. Dickson  & Michael D. Mendenhall  & 

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© 2004 Humana Press Inc., Totowa, NJ

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Brandt, P.C., Vanaman, T.C. (2004). Studies of Calmodulin-Dependent Regulation. In: Dickson, R.C., Mendenhall, M.D. (eds) Signal Transduction Protocols. Methods in Molecular Biology, vol 284. Humana Press. https://doi.org/10.1385/1-59259-816-1:111

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  • DOI: https://doi.org/10.1385/1-59259-816-1:111

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-245-2

  • Online ISBN: 978-1-59259-816-8

  • eBook Packages: Springer Protocols

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