Abstract
Blot overlay is a useful method for studying protein-protein interactions. This technique involves fractionating proteins on SDS-PAGE, blotting to nitrocellulose or PVDF membrane, and then incubating with a probe of interest. The probe is typically a protein that is radiola-beled, biotinylated, or simply visualized with a specific antibody. When the probe is visualized via antibody detection, this technique is often referred to as “Far Western blot.” Many different kinds of protein-protein interactions can be studied via blot overlay, and the method is applicable to screens for unknown protein-protein interactions as well as to the detailed characterization of known interactions.
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© 2004 Humana Press Inc., Totowa, NJ
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Hall, R.A. (2004). Studying Protein-Protein Interactions via Blot Overlay or Far Western Blot. In: Fu, H. (eds) Protein-Protein Interactions. Methods in Molecular Biology, vol 261. Humana Press. https://doi.org/10.1385/1-59259-762-9:167
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DOI: https://doi.org/10.1385/1-59259-762-9:167
Publisher Name: Humana Press
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