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Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions

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Book cover Bacterial Artificial Chromosomes

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 255))

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Abstract

Mammalian genome analysis has been advanced considerably by the development of yeast artificial chromosome (YAC) and bacterial artificial chromosome (BAC) cloning systems (1,2). These techniques have made the isolation of large, random DNA fragments possible, thereby greatly simplifying the physical mapping of chromosomes. However, isolation of entire genes and specific chromosomal regions for functional studies remained a laborious process because it relies on characterization of random clones in libraries comprising YACs or BACs. Even if a YAC or BAC contains an entire gene, the specific recovery of the gene was an arduous process. Often a gene is available as a contiguous set of fragments that must be pieced together.

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Author information

Authors and Affiliations

  1. Laboratory of Biosystems and Cancer, National Cancer Institute, Bethesda, MD

    Natalay Kouprina, Vladimir N. Noskov, Maxim Koriabine & Vladimir Larionov

  2. Department of Biology, Dong-A University, Pusan, Korea

    Sun-Hee Leem

Authors
  1. Natalay Kouprina
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  2. Vladimir N. Noskov
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  3. Maxim Koriabine
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  4. Sun-Hee Leem
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  5. Vladimir Larionov
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Editor information

Editors and Affiliations

  1. The Institute for Genomic Research, Rockville, MD

    Shaying Zhao

  2. Office of Biological and Environmental Research, US Department of Energy, Germantown, MD

    Marvin Stodolsky

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© 2004 Humana Press Inc., Totowa, NJ

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Kouprina, N., Noskov, V.N., Koriabine, M., Leem, SH., Larionov, V. (2004). Exploring Transformation-Associated Recombination Cloning for Selective Isolation of Genomic Regions. In: Zhao, S., Stodolsky, M. (eds) Bacterial Artificial Chromosomes. Methods in Molecular Biology™, vol 255. Humana Press. https://doi.org/10.1385/1-59259-752-1:069

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  • DOI: https://doi.org/10.1385/1-59259-752-1:069

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-988-9

  • Online ISBN: 978-1-59259-752-9

  • eBook Packages: Springer Protocols

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