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Identification of Proteins by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Peptide and Fragment Ion Masses

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2-D Proteome Analysis Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 112))

Abstract

Methods for the identification of proteins have advanced dramatically this decade through the introduction of mass spectrometric techniques and instrumentation sensitive enough to be applicable to biological systems (1). The two mass spectrometric techniques that have provided these advantages are electrospray-ionization mass spectrometry (ESI-MS) and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) (see Chapters 52, 54, and 55).

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References

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Author information

Authors and Affiliations

  1. Protein Structure, Amgen, Inc., Thousand Oaks, CA

    Paul L. Courchesne & Scott D. Patterson

Authors
  1. Paul L. Courchesne
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  2. Scott D. Patterson
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Editor information

Editors and Affiliations

  1. Department of Molecular Biotechnology, University of Washington, Seattle, WA

    Andrew J. Link

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© 1999 Humana Press Inc., Totowa, NJ

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Courchesne, P.L., Patterson, S.D. (1999). Identification of Proteins by Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Using Peptide and Fragment Ion Masses. In: Link, A.J. (eds) 2-D Proteome Analysis Protocols. Methods in Molecular Biology, vol 112. Humana Press. https://doi.org/10.1385/1-59259-584-7:487

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  • DOI: https://doi.org/10.1385/1-59259-584-7:487

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-524-9

  • Online ISBN: 978-1-59259-584-6

  • eBook Packages: Springer Protocols

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