Abstract
Mycobacteria possess a unique cell wall that mainly consists of a copolymer of peptidoglycan, arabinogalactan, and mycolic acids (1). The mycolic acids constitute the inner layer of an outer membrane with an exceptionally low fluidity (2) and permeability for hydrophilic compounds (3,4). Porins are water-filled channel proteins that mediate the transport of hydrophilic nutrients such as sugars and amino acids across the outer membrane of Gram-negative bacteria (5,6). A decade ago, channel proteins functionally similar to the porins of Gram-negative bacteria were discovered in mycobacteria (7,8).
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© 2003 Humana Press Inc., Totowa, NJ
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Heinz, C., Roth, E., Niederweis, M. (2003). Purification of Porins from Mycobacterium smegmatis . In: Selinsky, B.S. (eds) Membrane Protein Protocols. Methods in Molecular Biology, vol 228. Humana Press. https://doi.org/10.1385/1-59259-400-X:139
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DOI: https://doi.org/10.1385/1-59259-400-X:139
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