Abstract
The Epstein-Bar virus (EBV)-encoded complementary strand transcripts (CSTs) are a recently described (1,2) group of related transcripts initiating from a common promoter that exhibit a complex splicing pattern and contain a number of small open reading frames with the potential to encode a number of proteins (3). The CSTs have been detected in most types of EBV infection, including nasopharyngeal carcinoma (NPC) (4), oral hairy leukoplakia (5), EBV-positive gastric carcinoma (6), and latently infected lymphoblastoid cell lines (7,8). The function of these transcripts is still unclear.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Hitt, M. M., Allday, M. J., Hara, T., Karran, L., Jones, M. D., Busson, P., et al. (1989) EBV gene expression in an NPC related tumor. EMBO J. 8, 2639–2651.
Gilligan, K., Sato, H., Rajadurai, P., Busson, P., Young, L., Rickinson, A., et al. (1990) Novel transcription from the Epstein-Barr virus terminal EcoRI fragment, DIJhet, in a nasopharyngeal carcinoma. Virology 64, 4948–4956.
Smith, P. R., Gao, Y., Karran, L., Jones, M. D., Snudden, D., and Griffin, B. E. (1993) Complex nature of the major viral polyadenylated transcripts in Epstein-Barr virus associated tumors. J. Virol. 67, 3217–3225.
Chen, H.-L., Lung, M. M. L., Sham, J. S. T., Choy, D. T. K., Griffin, B. E., and Ng, M. H. (1992). Transcription of BamHI-A region of the EBV genome in NPC tissues and B cells. Virology 191, 193–201.
Lau, R., Middledorp, J., and Farrell, P. J. (1993). Epstein Barr virus gene expression in oral hairy leukoplakia. Virology 195, 463–474.
Sugiura, M., Imai, S., Tokunga, M., Koizumi, S., Uchizawa, M., Okamoto, K., and Osato, T. (1996). Transcriptional analysis of Epstein-Barr virus gene expression in EBV-positive gastric carcinoma: unique viral latency in the tumor cells. Br. J. Cancer 74, 625–631.
Karran, L., Gao, Y., Smith, P. R., and Griffin, B. E. (1992) Expression of a family of EB virus complementary strand transcripts in latently infected cells. Proc. Natl. Acad. Sci. USA 89, 8058–8062.
Brooks, L. A., Lear, A. L., Young, L. S., and Rickinson, A. B. (1993). Transcripts from the Epstein-Barr virus BamHI A fragment are detectable in all three forms of virus latency. J. Virol. 67, 3182–3190.
Sadler, R. H. and Raab-Traub, N. (1995). Structural analyses of the Epstein-Barr virus BamHI A transcripts. J. Virol. 69, 1132–1141.
Busson, P., Ganem, G., Flores, P., Mugneret, B., Clauss, B., Caillou, K., et al. (1988). Establishment and characterization of three transplantable EBV-containing nasopharyn-geal carcinomas. Int. J. Cancer 42, 599–606.
Allday, M. J. and Jones, M. D. (1987) Rapid processing of nitrocellulose filter lifts of bacteriophage lambda libraries. Nucleic Acids Res. 15, 10592.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2001 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Smith, P.R. (2001). Construction of cDNA Libraries for the Analysis of the Structure of Complementary Strand Transcripts (CSTs). In: Wilson, J.B., May, G.H.W. (eds) Epstein-Barr Virus Protocols. Methods in Molecular Biology™, vol 174. Humana Press. https://doi.org/10.1385/1-59259-227-9:37
Download citation
DOI: https://doi.org/10.1385/1-59259-227-9:37
Publisher Name: Humana Press
Print ISBN: 978-0-89603-690-1
Online ISBN: 978-1-59259-227-2
eBook Packages: Springer Protocols