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Electron Cryomicroscopy and Computer Image Processing Techniques

Use in Structure-Function Studies of Rotavirus

  • Protocol
Book cover Rotaviruses

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 34))

Abstract

Rotavirus (RV), a double-stranded (ds)RNA virus in the family Reoviridae, is a complex, relatively large (diameter, including spikes = 1000 Å), nonenveloped icosahedral virus. Once RV was recognized as a major human pathogen, it was extensively studied using modern molecular genetic and biological techniques, as discussed elsewhere in this book. These studies provided basic information about gene-coding assignments, protein processing, genome expression and replication, viral morphogenesis, and pathogenesis (1). In addition, molecular epidemiological studies, coupled with the characterization of neutralizing monoclonal antibodies (MAbs) and sequencing of the genes that encode the neutralizing antigens, provided an understanding at the molecular level of the antigenic and genetic variability of the RVs.

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Authors and Affiliations

  1. Verna and Marrs Department of Biochemistry and W. M. Keck Center for Computational Biology, Baylor College of Medicine, Houston, TX

    B. V. Venkataram Prasad

  2. Division of Molecular Virology, Baylor College of Medicine, Houston, TX

    Mary K. Estes

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  1. B. V. Venkataram Prasad
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  1. Clinical Microbiology and Public Health Laboratory Addenbrooke’s Hospital, Cambridge, UK

    James Gray  & Ulrich Desselberger  & 

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Venkataram Prasad, B.V., Estes, M.K. (2000). Electron Cryomicroscopy and Computer Image Processing Techniques. In: Gray, J., Desselberger, U. (eds) Rotaviruses. Methods in Molecular Medicine™, vol 34. Humana Press. https://doi.org/10.1385/1-59259-078-0:9

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  • DOI: https://doi.org/10.1385/1-59259-078-0:9

  • Publisher Name: Humana Press

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