Abstract
RNA localization is an important cellular mechanism for restricting the activities of regulatory proteins to cytoplasmic domains in oocytes and embryos (1). Cloning of these localized RNAs is obviously the first step in determining the functions of the encoded proteins. However, localized RNAs are rare and in low abundance. Their efficient isolation requires a combination of procedures to enrich for these RNAs.
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© 2000 Humana Press Inc.
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Zhang, J., Lou King, M. (2000). PCR-Based Cloning of Cortically Localized RNAs from Xenopus Oocytes. In: Tuan, R.S., Lo, C.W. (eds) Developmental Biology Protocols: Volume II. Methods in Molecular Biology™, vol 136. Humana Press. https://doi.org/10.1385/1-59259-065-9:309
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DOI: https://doi.org/10.1385/1-59259-065-9:309
Publisher Name: Humana Press
Print ISBN: 978-0-89603-853-0
Online ISBN: 978-1-59259-065-0
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