Abstract
Evanescent wave immunobiosensors use antibodies immobilized at the surface of a waveguide to form a sensing device. Fluorescence-based evanescent wave biosensors form a fluorescent complex at the surface of the waveguide when the antigen of interest is present (e.g., a sandwich assay or direct binding of a fluorescent analyte) or alternatively, the nonfluorescent analyte may compete with a fluorescent analog of the analyte for binding, as in a competitive immunoassay format. Light propagating through the waveguide extends a short distance into the surrounding medium and excites the immobilized fluorophore. The waveguide also collects the emitted fluorescence and carries this light to a photodetector.
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Wadkins, R.M., Ligler, F.S. (1998). Immunobiosensors Based on Evanescent Wave Excitation. In: Rogers, K.R., Mulchandani, A. (eds) Affinity Biosensors. Methods in Biotechnology, vol 7. Humana Press. https://doi.org/10.1385/0-89603-539-5:77
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DOI: https://doi.org/10.1385/0-89603-539-5:77
Publisher Name: Humana Press
Print ISBN: 978-0-89603-539-3
Online ISBN: 978-1-59259-485-6
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