Abstract
Standard PCR protocols can be used to amplify sequences of a few hundred base pairs in length from the DNA of a single cell (1,2). However, the efficiency of standard PCR amplification using Taq DNA polymerase declines with sequence length, making it difficult to amplify long sequences from small amounts of genomic DNA.
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© 1997 Humana Press Inc.
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Forrester, H.B., Radford, I.R. (1997). Amplification of DNA Sequences Up To 5 kb from Small Amounts of Genomic DNA Using Tub DNA Polymerase. In: White, B.A. (eds) PCR Cloning Protocols. Methods in Molecular Biology™, vol 67. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-483-6:31
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DOI: https://doi.org/10.1385/0-89603-483-6:31
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-483-9
Online ISBN: 978-1-59259-553-2
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