Abstract
Standard protocols for definite species identification of nontuberculous mycobacteria by phenotypic characteristics include complex and time-consuming procedures. Other methods based on lipid analysis, such as high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and gas-liquid chromatography (GLC), are cumbersome, expensive, and are used in only a few clinical laboratories.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Bbttger, E C, Teske, A., Knschner, P., Bost, S., Chang, H. R., Beer, V., and Hirschel, B. (1992) Disseminated ’Mycobacterium genavense mfection in patients with AIDS. Lancet 340, 76–80.
Horsburgh, C. R. (1991) Mycobacterium avium complex mfections in the acquired immunodeficiency syndrome. N. Engl. J. Med. 324, 1332–1338.
Butler, W. R., O’Connor, S. P, Yakrus, M. A., and Gross, W. M (1994) Cross-reactivity of genetic probe for detection of Mycobacterwm tuberculosis with the newly described species Mycobacterrum celatum J Clm. Microblol 32, 536–538
Bull, T J., Shanson, D C, and Archard, L C. (1995) Rapid identificatton of mycobacteria from AIDS patients by capillary electrophoretic profiling of amphfied SOD gene J Clin Pathol. Mol. Pathol. 48, 124–132
Fiss, E. H, Chehab, F F., and Brooks, G. F. (1992) DNA amplification and reverse dot blot hybridization for detection and identtficatton of mycobacterta to the species level in the clmical laboratory. J Clan Mzcroblol 30, 1220–1224.
Hughes, M. S, Skuce, R A., Beck, L.-A, and Nell, S. D (1993) Identification of mycobacteria from animals by restriction enzyme analysis and direct DNA cycle sequencing of polymerase chain reaction-amplified 16s rRNA gene sequences J Clin Mxroblol. 31, 3216–3222
Mabilat, C., Desvarenne, S., Pantetx, G., Machabert, N., Bernillon, M.-H., Guardlola, G, and Cros, P. (1994) Routine identification of Mycobacterium tuberculosts complex isolates by automated hybrtdization. J Clan Mzcrobrol 32, 2702–2705.
Semi, H., Bottger, E C, and VilJanen, M K (1994) Identification of mycobacteria by PCR-based sequence determination of a 32-kilodalton protein gene J Clin Mxroblol 32, 2944–2947
Takewaki, S.-I., Okuzumi, K., Manabe, I., Tanimura, M., Miyamura, K., Nakahara, K.-I., Yazaki, Y., Ohkubo, A, and Nagai, R (1994) Nucleotide sequence comparison of the mycobacterial dnaJ gene and PCR-restrtctton fragment length polymorphism analysis for identification of mycobacterial species Int J Syst Bactenol 44, 159–166
Telenti, A., Marchest, F., Balz, M., Bally, F., Bottger, E C., and Bodmer, T (1993) Rapid identification of mycobacteria to the species level by polymerase chain reaction and restrtctton enzyme analysis. J Clin Microbiol 31, 175–178
Bottger, E C. (1996) Approaches for identification of microorganisms ASM News 62, 247–250
Rogall, T, Flohr, T., and Bottger, E C. (1990) Differentiatton of mycobacterium species by direct sequencing of amplified DNA. J Gen Mxroblol 136, 19151920
Woese, K. H. (1987) Bacterial evolutionMicrobiol. Rev. 51, 221–271.
Ktrschner, P., Springer, B., Vogel, U., Merer, A, Wrede, A., Kiekenbeck, M, Bange, F.-C., and Bottger, E. C. (1993) Genotypic identification of mycobacteria by nucleic acid sequence determination: report of a 2-year experience in a clmical laboratory. J Clin Mzcroblol. 31, 2882–2889.
Kirschner, P., Meter, A., and Bottger, E. C. (1993) Genotypic identificatton and detection of mycobacteria. facing novel and uncultured pathogens, in Diagnostic Molecular Microbiology. (Persing, D. H., Smith, T. F., Tenover, F. C., and White, T J, eds.), American Society for Microbiology, Washinghton, DC, pp. 173–190
Springer, B, Stockman, L, Teschner, K, Roberts, G D, and Bottger, E C (1996) Two-laboratory collaborative study on identification of mycobacteria molecular versus phenotyptc methods J Clin Mzcrobiol 34, 296–303
Maidak, B. L., Olsen, G. J., Larsen, N., Overbeek, R., McCaughey, M. J., and Woese, C. R (1996) The ribosomal database project (RDP) Nucleic Aczd Res 24, 82–85.
Boddinghaus, B., Rogall, T., Flohr, T., Blocker, H., and Bottger, E C. (1990) Detection and identification of mycobacteria by amplification of rRNA J Clan. Mzcrobml. 28, 1751–1759.
Hultman, T., Stahl, S., Hornes, E., and Uhlen, M (1989) Direct solid phase sequencing of genomlc and plasmid DNA using magnetic beads as support. Nucleic Acids Res 17, 4837–4946
Kirschner, P., Rosenau, J., Springer, B., Teschner, K., Feldmann, K, and Bottger, E. C. (1996) Diagnosis of mycobacterial infections by nucleic acid amplification. 18-month prospective study. J. Ch Microbiol 34, 304–312.
Fickett, J W (1996) Finding genes by computer. the state of the art. Trends Genet. 12, 316–320
Kirschner, P., Vogel, U., Hein, R., and Bottger, E. C. (1993) Bias of culture techniques for diagnosing mixed Mycobacterium genavense and Mycobacterium avium mfectton in AIDS. J Clin Mlcrobiol. 32, 828–831
Hofer, M, Hirschel, B., Kirschner, P., Beghetti, M., Kaehm, A., Sregrrst, C. A., et al. (1993) Disseminated osteomyelitis from Mycobacterium ulcerans after a snakebite. N Engl J Med 328, 1007–1009.
Kirschner, P and Bottger, E C. (1992) Microheterogeneity within rRNA of Mycobacterium gordonae. J Clm Microbial. 30, 1049.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc., Totowa, NJ
About this protocol
Cite this protocol
Kirschner, P., Bottger, E.C. (1998). Species Identification of Mycobacteria Using rDNA Sequencing. In: Parish, T., Stoker, N.G. (eds) Mycobacteria Protocols. Methods in Molecular Biology™, vol 101. Humana Press. https://doi.org/10.1385/0-89603-471-2:349
Download citation
DOI: https://doi.org/10.1385/0-89603-471-2:349
Publisher Name: Humana Press
Print ISBN: 978-0-89603-471-6
Online ISBN: 978-1-59259-576-1
eBook Packages: Springer Protocols