Abstract
The mucosal endocrine cells in the antrum are found as individual elements interspersed among the other epithelial cells (i.e., mucin cells) (Fig. 1). In order to establish the factors regulating endocrine cell function, these cells have to be separated not only from the surrounding epithelial cells, but also from circulating and neuronal elements within the stomach.
A section of human antrum immunostained using the peroxidase method to localize the gastrin-immunoreactive endocrine cells (arrows). Note that the cells are grouped in the mid-third of the mucosa with no immunoreactive cells at the top of the gastric pits (GP) (×100).
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References
Soll, A, Amirian, D, Park, J, Elashoff, J, and Yamada, T (1985) Cholecystokinin potently releases somatostatin from canine fundic mucosal cells in short-term culture Am J Physiol 248, G569–G573
Buchan, A M J and Meloche, R M (1994) Signal transduction events involved in bombesin-stimulated gastrin release from human G cells in culture Can J Physiol Pharmacol 72, 1060–1065
Buchan, A M J, Curns, S B., and Meloche, R M (1990) Release of somatostatinimmunoreactivity from human antral D cells III culture Gustroenterology 99, 690–696
Buchan, A M J, Meloche, M, and Coy, D H (1990) Inhibition of bombesinstimulated gastrin release from isolated G cells by bombesm analogs Pharmacology 41, 237–245
Buchan, A M J (1991) Effect of sympathomimetics on gastrin secretion from antral cells in culture J Clan Invest 87, 1382–1386
Buchan, A M J, MacLeod, M D, Meloche, R M, and Kwok, Y N (1992) Muscarinic regulation of somatostatm release from primary cultures of human antral epithelial cells Pharmacology 44, 33–40.
Buchan, A M J, Meloche, R M., Kwok, Y N, and Kofod, H (1993) Effect of CCK and secretin on somatostatin release from cultured antral cells Gastroenterology 104, 1414–1419
Campos, R V, Buchan, A M J, Meloche, M., Pederson, R A, Kwok, Y N, and Coy, D H (1990) Gastrin secretion from isolated human G cells in primary culture Gastroenterology 99, 36–44
Guarnieri, M, Krell, L S, McKhann, G M., Pasternak, G W, and Yamamura, H I (1975) The effects of cell isolation techniques on neuronal membrane receptors Brain Res 93, 337–342
Meinstrich, M L (1983) Experimental factors involved in separation by centrifugal elutriation, in Cell Separation Methods and Selected Applications, vol 2 (Pretlow, T G and Pretlow, T P, eds), Academic, New York, pp 33–61
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© 1996 Humana Press Inc., Totowa, NJ
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Buchan, A.M.J. (1996). Primary Culture of Human Antral Endocrine Cells. In: Jones, G.E. (eds) Human Cell Culture Protocols. Methods in Molecular Medicine, vol 2. Humana Press. https://doi.org/10.1385/0-89603-335-X:345
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DOI: https://doi.org/10.1385/0-89603-335-X:345
Publisher Name: Humana Press
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