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Use of DNA- Halo Preparations for High- Resolution DNA In Situ Hybridization

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In Situ Hybridization Protocols

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 33))

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Abstract

The past 10 years have witnessed an increasing application of in situ hybridization techniques in biomedical research. This is mainly a result of the development of nonradioisotopic nucleic acid hybridization detection formats. Such methods allow much better exploitation of the spatial resolution provided by light microscopy than radioisotopic detection formats such as microautoradiography (1, 2). Also the spectral resolution of light microscopy can be used to advantage with such methods, because they readily enable the simultaneous detection of multiple nucleic acid sequence targets on the basis of different colors (3–5). In situ hybridization techniques are now accepted as powerful research tools in molecular cell biology and genetics (for reviews see refs. (6–8).

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Authors and Affiliations

  1. Department of Cytochemistry and Cytometry,Medical Faculty, Leiden University, Leiden, The Netherlands

    Anton K. Raap & Joop Wiegant

Authors
  1. Anton K. Raap
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  2. Joop Wiegant
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Editor information

Editors and Affiliations

  1. Murdoch Institute, Melbourne, Australia

    K. H. Andy Choo

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© 1994 Humana Press Inc.

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Raap, A.K., Wiegant, J. (1994). Use of DNA- Halo Preparations for High- Resolution DNA In Situ Hybridization. In: Choo, K.H.A. (eds) In Situ Hybridization Protocols. Methods in Molecular Biology™, vol 33. Humana Press. https://doi.org/10.1385/0-89603-280-9:123

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  • DOI: https://doi.org/10.1385/0-89603-280-9:123

  • Publisher Name: Humana Press

  • Print ISBN: 978-0-89603-280-4

  • Online ISBN: 978-1-59259-520-4

  • eBook Packages: Springer Protocols

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