Abstract
In the previous chapter we described linker scanning mutagenesis by oligonucleotide ligation. In this chapter we describe a more recent and versatile procedure that makes use of amplification by the polymerase chain reaction (PCR). For a description of the traditional method of generating linker scanning mutants by combination of deletion fragments, see ref. 1.
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© 1994 Humana Press Inc, Totowa, NJ
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Harlow, P.P., Hobson, G.M., Benfield, P.A. (1994). Construction of Linker-Scanning Mutations Using the Polymerase Chain Reaction. In: Harwood, A.J. (eds) Protocols for Gene Analysis. Methods in Molecular Biology, vol 31. Humana Press. https://doi.org/10.1385/0-89603-258-2:87
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DOI: https://doi.org/10.1385/0-89603-258-2:87
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