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Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry

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Biomembrane Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 27))

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Abstract

An important topic in membrane biochemistry is the determination of the sidedness of the components comprising a biological membrane. Neither phospholipids nor membrane proteins are distributed symmetrically over inner and outer aspects of cellular membranes, and knowledge of the topological distribution is a prerequisite for understanding the functional properties of membranes. This chapter will deal with a recently developed, noninvasive technique to assess the distribution of phospholipids over the two leaflets of biological membranes with emphasis on the distribution of negatively charged phospholipids (1,2).

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Authors and Affiliations

  1. Department of Biochemistry, Cardiovascular Research Institute Maastricht, University of Limburg, Maastricht, The Netherlands

    Paul Comfurius, Edouard Bevers & Robert F. A. Zwaal

Authors
  1. Paul Comfurius
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  2. Edouard Bevers
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  3. Robert F. A. Zwaal
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© 1994 Humana Press Inc. Totowa, NJ

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Comfurius, P., Bevers, E., Zwaal, R.F.A. (1994). Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry. In: Biomembrane Protocols. Methods in Molecular Biology, vol 27. Springer, Totowa, NJ. https://doi.org/10.1385/0-89603-250-7:131

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  • DOI: https://doi.org/10.1385/0-89603-250-7:131

  • Publisher Name: Springer, Totowa, NJ

  • Print ISBN: 978-0-89603-250-7

  • Online ISBN: 978-1-59259-514-3

  • eBook Packages: Springer Protocols

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