Abstract
Immunoaffinity chromatography using immobilized monoclonal antibodies has become one of the most powerful macromolecular separation methods available. In many cases, simple one-step immunoaffinity separations will produce a several thousand-fold purification with yields of better than 80%. Monoclonal antibodies are now available with specificities for many different antigens, and the technology for producing new monoclonal antibodies has become widespread througout the world since its invention (1; see vol. 1 of this series). It has been estimated that a new monoclonal antibody is produced every 40 min worldwide.
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Kenney, A., Goulding, L., Hill, C. (1988). The Design, Preparation, and Use of Immunopurification Reagents. In: Walker, J.M. (eds) New Protein Techniques. Methods in Molecular Biology™, vol 3. Humana Press. https://doi.org/10.1385/0-89603-126-8:99
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DOI: https://doi.org/10.1385/0-89603-126-8:99
Publisher Name: Humana Press
Print ISBN: 978-0-89603-126-5
Online ISBN: 978-1-59259-490-0
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