Effects of xenogeneic acellular bone on the astrocyte cellsChinese Full Text
LI Li-hua;ZHANG-Yu;HUANG Jin-long;LI Mei;XIA Hong;HOU Li;Hospital of Orthopedics,General Hospital of Guangzhou Military Command of PLA;
Abstract: Objective To observe the effects of xenogeneic acellular bone on the proliferation,migration and characteristic of astrocyte cells.Methods The xenogeneic bone was prepared by epoxy crosslinking technology after chemical and physical processing.Different concentrations（0.05,0.1,0.2 g /mL） of xenogeneic bone extracted liquid were added into the culture mediums with astrocyte cells.Scanning electron microscope（SEM） was used for morphologic observation of astrocyte cells.MTT method was used detect the proliferation of the astrocyte cells,and to optimize the concentration of xenogeneic bone extracted liquid.Transwell method and flow cytometry were used to access the cell migration and cell cycle,respectively.Results The astrocyte cells were polygonal,and were in a good state under SEM.The optimized concentration of xenogeneic bone extracted liquid was 0.05 g /mL,which had a positive effect on cell proliferation.In this concentration,the number of transwell cells was increased（P ＜ 0.05）,with no alteration on the cell cycle（P ＞0.05）.Conclusion Xenogeneic acellular bone has low influence on astrocyte cells,providing good biosecurity to astrocyte cells.
- DOI：
10.13820/j.cnki.gdyx.2014.10.002
- Series：
- Subject：
- Classification Code：
R329.2
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