显色原位杂交技术及其在临床病理中的应用
李瑶田林曹书芬度长海张丽
湖北医药学院附属人民医院病理科
Abstract: ＜正＞显色原位杂交（chromogenic in situ hybridization,CISH）是一种近几年来替代荧光原位杂交（Fluorescence in situ hybridization,FISH）用以检测基因扩增的新方法。它最早于2000年应用于检测乳腺癌HER-2（human epidermal growth factor receptor type-2,HER-2）基因扩增,目前该技术已经日渐完善,并应用于胃癌、肺癌、结肠癌、淋巴瘤等疾病的实验室及临床研究。本文主要就CISH的基本原理、特点及其临床病理应用,与FISH方法进行比较做一综述。
- DOI：
10.13819/j.issn.1006-9674.2014.01.028
- Series：
- Subject：
- Classification Code：
R730.4
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