Chest
Clinical InvestigationsThe Differential Effect of Cigarette Smoke on the Growth of Bacteria Found in Humans
Section snippets
Bacterial Isolates Studies
All isolates studied were collected from mouth cultures and respiratory tract cultures of patients over several months. The isolates were stored frozen at — 70°C, and when needed, were thawed and grown on sheep blood tryptic soy agar or chocolate agar (Hemophilus species) before testing.
Preparation of Smoke Solution
Smoke from two brands of cigarettes (Kool or Camel) was drawn through 10 ml of tryptic soy broth (BBL, Cockeysville, Md) to produce a minimum steady stream of bubbles. The smoke-saturated solution was filter
RESULTS
With few exceptions, the Gram-positive cocci were more susceptible to growth inhibition by cigarette smoke than the Gram-negative bacilli (Fig 1). Pseudomonas aeruginosa, Escherichia coli, Klebsiella, Enterobacter, Serratia, Providencia, Citrobacter, and Acinetobacter were all resistant to a 1:1 dilution of the smoke solution. The Gram-positive cocci, with the exception of Enterococcus faecalis, were susceptible to growth inhibition even at dilutions as high at 1:8. The three fastidious
DISCUSSION
Tobacco has long been considered, especially in the premodern medical era, to have medicinal value. Tobacco and tobacco smoke have been used on infected body sites and have allegedly effected improvements if not cures.7 Many of the components of cigarette smoke have been identified, and compounds such as nicotine and acrolein have been shown to be present in high concentrations.8
The nicotine content in unfiltered cigarettes is in the range of 1 mg.9 The smoke from this cigarette may transfer a
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