CD8+ T cell cytotoxicity mediates pathology in the skin by inflammasome activation and IL-1β production
Fig 4
Treatment of mice with NLRP3 inhibitors dampens the immunopathology caused by CD8 T cells.
WT C57BL/6 mice were infected with L. major in the ear, and 2 weeks later mice were co-infected with 2×105 PFU of LCMV Armstrong strain by i.p. injection. Ten days post LCMV infection mice were treated with MCC950, glyburide or vehicle; (a and b) ear thickness was assessed weekly. Five weeks post infection with L. major, mice were euthanized and the lesions were digested and the (c and d) frequency of neutrophils in the skin was determined directly ex vivo by flow cytometry. (e and f) Number of parasites in the skin was determined at 5 weeks post infection with L. major. Results in mice are data from one experiment with 5 mice per group. *p ≤ 0.05, **p<0.01 or ***p ≤ 0.001