A single mutation in the envelope protein modulates flavivirus antigenicity, stability, and pathogenesis
Fig 9
Effect of natural antibodies on WNV intrinsic decay.
Representative intrinsic decay curves of fully infectious WT and T198F viruses in serum obtained from (A) naïve WT C57BL/6J mice, (B) naïve μMT C57BL/6J mice, and (C) media. Experiments were performed as described in Fig 4A. Error bars indicate the SEM from triplicate infections. (D) Average half-life values of WT and T198F viruses following prolonged incubation in serum obtained from naïve WT mice, naïve μMT mice, or media obtained from two independent experiments performed using independent serum samples and virus preparations. Error bars indicate the SEM. Fold-differences in half-life between WT and T198F WNV in each incubation condition are indicated.