MyD88 Shapes Vaccine Immunity by Extrinsically Regulating Survival of CD4+ T Cells during the Contraction Phase
Fig 1
Myd88-/- mice fail to mount Th17 and Th1 cells and resistance after vaccination.
Wild type C57BL6 and Myd88-/- mice were s.c. vaccinated with 105 or 106 live B.dermatitidis yeast and followed for survival (A). To circumvent dissemination, mice were vaccinated s.c. with 106 heat-killed yeast and challenged i.t. with 2 x 103 26199 yeast. Two weeks post-infection, lung CFU were enumerated (B). Data are the mean ± SEM (n = 8–10 mice/group). Data are representative of three independent experiments. The number of Th17 and Th1 cells recalled to the lung were assessed at day 4 post-infection. Data are the mean ± SEM (n = 4–6 mice/group) (C). The numbers indicate the mean ± SEM of cytokine producing CD4+ T cells per mouse. * P <0.05 vs. vaccinated wild-type controls. Dot plots show concatenated samples of 4–6 mice/ group. Data are representative of three independent experiments. (D) Wild-type and Myd88-/- mice were vaccinated s.c. with 107 live H. capsulatum yeast and challenged with 5 x 105 Hc G217B yeast. Two weeks post-infection, lung CFU were enumerated. * P <0.05 vs. vaccinated wild-type controls. (E) The frequencies of Th17 and Th1 cells were enumerated at day 4 post-infection. Data are the mean ± SEM (n = 4–6 mice/group).