Intraperitoneal Infection of Wild-Type Mice with Synthetically Generated Mammalian Prion
Fig 5
Ultrastructural changes in rec-Prion challenged CD-1 mice detected by immunogold electron microscopy.
(a) and (b) show dendritic membrane invaginations (arrows) in two dendrites (D). The reflected membrane invagination is labelled for PrP-d. The membrane invaginations are branched and the ends of the branches show a membrane coating on the cytoplasmic face of the membrane (arrowheads). Bars = 500 nm. (c) shows two weakly PrP-d labelled spiral membrane invaginations with continuous membrane undercoated buds in an axon terminal containing numerous synaptic vesicles (S). The spiral nature of the invaginations can be seen between arrowheads. One invagination is tangentially sectioned and lies apparently free within the cytoplasm. The other is visibly connected to the membrane (arrow). Bar = 500nm. (d) shows immunogold labelling for PrP-d within a lysosome (L) of a microglial process (m). Bar = 1 μm. All images were taken from medulla of i.p. challenged terminal disease affected mouse and 1A8 antibody was used to perform the immunogold detection of PrP-d.