The Expression of Functional Vpx during Pathogenic SIVmac Infections of Rhesus Macaques Suppresses SAMHD1 in CD4+ Memory T Cells
Fig 8
Mapping of Vpx mutations at the Vpx-DCAF1-SAMHD1 interface.
(A) Crystal structure (PDB ID 4CC9) of the c-terminal domain of SAMHD1 (green) in complex with WT Vpx (blue) and DCAF1 (salmon). (B) Zoomed-in view displaying the interactions of Gln 76Vpx at the Vpx-DCAF1 interface. Models of the Q76A Vpx mutation (C), the Q76S Vpx substitution (D) in the revertant recovered from macaque K42, and the Q76A/I32T Vpx double substitution in the revertant recovered from macaque JAX4 (E). Hydrogen bonds are indicated by dashed lines and the red spheres denote a crystallographic water molecule observed the structure.