Varicella Viruses Inhibit Interferon-Stimulated JAK-STAT Signaling through Multiple Mechanisms
Fig 6
ORF63 induces proteasomal-degradation of IRF9, but does not affect IRF1 or IRF3.
(A) TRFs were infected with AdORF63 at MOI 20 and AdTA at MOI 10 in the presence of 1000 ng/ml Dox (-) or 1 ng/ml Dox (+) for 48 hours. During the last 16 hours of infection cells were incubated with increasing concentrations the proteosomal inhibitor MG132. Lysates were stained for IRF9 and ORF63 levels by SDS-PAGE ans western blot. The GAPDH signal was used a loading control. (B) The ratio of IRF9 to GADH expression in MG132-treated (10 μM) control and ORF63-expressing cells is presented. Shown are the means ± standard error of the average of 4 independent experiments, * indicate P-value of <0.01. (C) TRFs were infected with AdORF63 at MOI 20 and AdTA at MOI 10 in the presence of the indicated amounts of Dox. At 48 hours p.i. cells were lysed and stained for the indicated proteins using specific antibodies. The GAPDH signal was used a loading control.