HITS-CLIP Analysis Uncovers a Link between the Kaposi’s Sarcoma-Associated Herpesvirus ORF57 Protein and Host Pre-mRNA Metabolism
Fig 5
Sequence traces of ORF57-bound human RNAs.
Each panel is a screen shot (IGV browser [105]) of the input and pellet samples from one biological replicate. The numbers at the top left of each frame are proportional to the number of reads stacked in a given window. The x-axes in each panel were scaled for each specific transcript. The black bars in the pellet panels denote positions of the enriched clusters and the asterisks denote approximate positions of characteristic mutations. Below each panel is a gene diagram with the TSS and gene orientation indicated by the black arrow. Green and blue arrows above and below the gene diagram indicate the positions of primers used to assay pre-mRNA and mRNAs, respectively. The exons (thick black rectangles) and introns (black lines) are to scale, but the primer and amplicon lengths are not. When possible, primers or amplicons for mRNAs were designed to span exon-exon boundaries (blue dashed lines).