Differential Reliance on Autophagy for Protection from HSV Encephalitis between Newborns and Adults
Figure 7
Infected regions with activated autophagy in the newborn mouse brain are associated with markers of apoptotic cell death.
(A) Representative immunohistochemical analysis of neonatal murine brain infected with WT HSV-1 F (original magnification: 200x). The HSV antiserum panel from Fig. 5 is repeated for orientation. The WT HSV-infected neonatal brain is positive for TUNEL staining and caspase-3 activation (CC-3) at the site of infection (top). Representative sections from a neonatal murine brain infected with the dBBD virus also demonstrate positive TUNEL staining (middle) and cleaved caspase-3 (middle, right) in infected regions of the brain. The control uninfected neonatal brain is negative for markers of apoptosis (bottom). (B) Serial sections of brain from an adult mouse infected with HSV-1 F demonstrate scant TUNEL staining and few detectable cleaved caspase-3 positive cells (top). The dBBD-infected adult murine brain is also scantly positive for TUNEL staining (middle), and cleaved caspase-3 was positive in serial sections. Control uninfected adult mouse brain sections are shown below (original magnification: 200x).