T-bet and Eomes Are Differentially Linked to the Exhausted Phenotype of CD8+ T Cells in HIV Infection
Figure 4
Polyfunctional characterization of virus-specific CD8+ T cells in untreated HIV-infected individuals.
(A) Distribution of PD-1+ (red) and PD-1− (blue) HIV-specific CD8+ T cells within the T-bet/Eomes axis and linkage to IFNγ and TNF, CD107a and Granzyme B expression. (B) Boolean combinations of all functional and inhibitory markers (n = 256) were combined using PCA, generating a HIV- (orange) and CMV-specific (green) PC1 and PC2 score for each HIV infected subject (n = 23). (C) Correlations between the MFI of Eomes and IFNγ, TNF, CD107a and Granzyme B or single CD107a secretion. (D) SPICE analysis of all functional combinations between the T-betdimEomeshi (red) and T-bethiEomesdim (blue) population for HIV-specific CD8+ T cells. Median and IQR are shown for all bars and Wilcoxon matched-pairs single rank tests were performed to compare outcomes between groups; *P<0.05. Functional combinations where the IQR did not exceed 1% are not depicted in the graph. Permutation test was performed between the pie charts.