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A Nucleic-Acid Hydrolyzing Single Chain Antibody Confers Resistance to DNA Virus Infection in HeLa Cells and C57BL/6 Mice

Figure 2

3D8 scFv expression in transgenic HeLa cells confers resistance to HSV infection.

A. Three 3D8 scFv cell lines and one mutant 3D8scFv cell line were challenged with HSV::GFP at different MOIs (0.1, 0.5, and 1). The SCH07072 line showed the lowest GFP expression compared to that of the other lines (SCH07041, SCH07071, and muSCH). B. Wild-type HeLa, SCH07072, and muSCH cells were infected with HSV::GFP at 0.1 and 0.5 MOI. Plaque assays revealed that SCH07072 cells had the lowest amount of virus progeny (0.1 MOI: HeLa = 1,343.33±139.68, SCH07041 = 266.67±31.80, SCH07071 = 483.33±43.72, SCH07072 = 163.33±29.06, muSCH = 1,356.67±131.70; 0.5 MOI: HeLa = 7,851.00±209.10, SCH07041 = 1,964.00±263.04, SCH07071 = 2,649.67±49.02, SCH07072 = 530.67±55.68, muSCH = 7,646.67±233.90). Data bars show mean ± standard error. *** indicate significant differences from HeLa cells at p<0.001 (one-way analysis of variance and Tukey's post hoc t-test). C. Western blot analysis using an anti-HSV DNA polymerase antibody demonstrated that less HSV DNA polymerase protein was present in SCH07072 cells compared to wild type HeLa cells 48 hr after virus challenge.

Figure 2

doi: https://doi.org/10.1371/journal.ppat.1004208.g002