The Highly Conserved Bacterial RNase YbeY Is Essential in Vibrio cholerae, Playing a Critical Role in Virulence, Stress Regulation, and RNA Processing
Figure 9
Complementation of E. coli ΔybeY by ybeY of various pathogens.
A) E. coli MC4100 Wt pY and the ΔybeY pY strain grown in LB medium were serially (1∶10) diluted and spotted onto LB plates containing various antibiotics or H2O2, treated with heat (45°C) or UV (60 J/m2) as indicated. “pY_Ec” refers to a plasmid expressing ybeY of E. coli, similarly V. cholerae N16961 (pY_Vc), P. aeruginosa PAO1 (pY_Pa), M. tuberculosis H37Rv (pY_Mt), and S. pneumoniae R6 (pY_Sp). B) Total RNA was isolated from E. coli MC4100 transformed with the empty vector, and the E. coli ΔybeY strain transformed with plasmids containing ybeY from various bacteria as indicated. RNA was analyzed by agarose gel electrophoresis. The positions of 23 S, 17 S, 16 S and 16 S* rRNAs are indicated based on their mobility.