The Triggering Receptor Expressed on Myeloid Cells 2 Inhibits Complement Component 1q Effector Mechanisms and Exerts Detrimental Effects during Pneumococcal Pneumonia
Figure 1
Pulmonary TREM-2 expression and function during S. pneumoniae induced inflammation.
(A) TREM-1 and TREM-2 expression was evaluated in the indicated cell types using RT-PCR. (B) Western blot was used to evaluate TREM-2 expression on AM. (C) WT mice (n = 6 per time point) were intranasally inoculated with 105 CFU S. pneumoniae and after indicated time points TREM-2 lung transcript levels were evaluated. (D) WT AM were treated with 2×107 CFU/ml S. pneumoniae for indicated time points and TREM-2 RT-PCR was conducted. (E–G) WT and Trem-2−/− mice (n = 7 per genotype) were intranasally infected with 105 CFU S. pneumoniae for 6 h and levels of indicated cytokines were evaluated in the lung (E) or BALF (F) and neutrophil counts were determined in the BALF (G). Data represent mean ± SEM and are (A–G) representative of two independent experiments. Differences were calculated versus time point 0 (C–D) or versus WT (E–G) and are indicated as * p<0.05, ** p<0.005, **** p<0.0001.