Prophenoloxidase Activation Is Required for Survival to Microbial Infections in Drosophila
Figure 9
Microbial persistence in wild-type and PPO1Δ, PPO2Δ double mutant flies.
Persistence of E. carotovora (A), S. typhimurium (B), L. monocytogenesis (C), E. faecalis (D), S. aureus (E) and C. albicans (F) were evaluated at different times post- infection in wild-type and PPO1Δ, PPO2Δ mutant flies (referred to as PPOΔ). Statistical analysis reveals no bacterial persistence difference in PPO1Δ, PPO2Δ double mutants compared to wild-type flies upon E. carotovora, S. typhimurium, L. monocytogenesis, E. faecalis and C. albicans infections. However, we noticed an increased counts of S. aureus in PPO1Δ, PPO2Δ double mutants compared to wild-type flies (1 day: p = 0.04113; 2 days: p = 0.002165; 3 days: p = 0.004922; 4 days: p = 0.009524). Bars = median. The number of colony forming units (CFU) is expressed per fly and in a logarithmic scale. Data were analyzed by Wilcoxon test and values are pooled from three independent experiments.