De novo Fatty Acid Biosynthesis Contributes Significantly to Establishment of a Bioenergetically Favorable Environment for Vaccinia Virus Infection
Figure 3
Palmitate is utilized within mitochondria for energy production via ß-oxidation.
A) One step growth analysis of BSC40 cells infected with WT vaccinia virus (MOI 5) in glucose-free media (gray bar) or glucose-free media supplemented with 4 mM glucose (black bar); viral yield at 16 hpi is shown (n = 6). B) One step growth analysis of BSC40 cells infected with WT vaccinia virus (MOI 5) in the presence (left bars) or absence (right bars) of 4 mM glucose supplemented with DMSO (vehicle control) (solid bars) or etomoxir (360 µM) (striped bars), an inhibitor of long-chain fatty acid import into the mitochondria; viral yield at 16 hpi is shown (n = 6) (***, p<0.001). C) One-step growth analysis of BSC40 cells infected with WT vaccinia virus (MOI 5) in the presence (black diamonds) or absence (gray circles) of 4 mM glucose and treated with various concentrations (0–8 mM) of trimetazidine, an inhibitor of ß-oxidation; viral yield at 16 hpi is shown (n = 6).