MAIT Cells Detect and Efficiently Lyse Bacterially-Infected Epithelial Cells
Figure 7
Experimental in vivo Shigella flexneri-infection results in MAIT cells activation.
MAIT cell phenotype was analyzed in PBMCs from volunteers vaccinated with an attenuated strain of Shigella dysenteriae 1 (SC599) and compared to subjects receiving a placebo. (A) Expression of the activation marker HLA-DR on MAIT cells at base line or D11. (B) MAIT cell percentages in the blood that have been normalized to the BL value of volunteers receiving a placebo (white symbols) or the vaccine. Each dot represents the value for a subject and the lines join the values obtained at the different time points. * indicates statistical significance between D7 and D11 by paired Wilcoxon t-test. (C) Upper panel: MAIT cell percentages of volunteers that have received the placebo or the vaccine segregated according to their B-cell response: Non Responders (grey symbols) and Responders (black symbols). Each dot represents the value for a subject and the lines join the values obtained at the different time points: at base line (BL), at day 7, 9 and 11 post vaccination. Lower panel: proportion of HLA-DR positive cells in the MAIT cell subset represented as above. * indicates statistical significance between BL and D11 by paired Wilcoxon t-test, or unpaired Mann-Whitney t-test between non-responder and responder at D11.