The DAF-16/FOXO Transcription Factor Functions as a Regulator of Epidermal Innate Immunity
Figure 5
The Gαq-PLCβ signaling is required for DAF-16 nuclear accumulation.
(A) egl-30(n686) or egl-8(n488) mutants displayed reduced the production of ROS induced by D. coniospora infection (DC) and spiny balls (SB). These results are means±SD of four experiments. * P<0.05 versus control (N2). (B) Quantification of DAF-16 distribution. n = 100–110 nematodes per condition. These results are means±SD of four experiments. *P<0.05 versus control (DC and SB). (C) Mutations in egl-30 and egl-8 inhibited the expression of Psod-3::GFP induced by D. coniospora infection and treatment with spiny balls or 12 h. *P<0.05 versus control (N2). (D–E) Mutations in elg-30 and egl-8 reduced the survival rate of worms exposed to D. coniospora (D) and spiny balls (E). P<0.001 relative to control with wild-type worms. However, mutations in egl-30 or egl-8 did not enhanced susceptibility of daf-16(mu86) mutants to killing by D. coniospora (D) and spiny balls (E).