Real-Time Whole-Body Visualization of Chikungunya Virus Infection and Host Interferon Response in Zebrafish
Figure 7
Neutrophils are key ifnφ1-producing cells.
(A–C) Effect of myeloid cell depletion (PU.1 MO, 3 independent experiments), (D–F) of macrophage depletion (metronidazole treatment of mpeg1:Gal4/UAS:NfsB-mCherry fish, 2 independent experiments), (G–I) of neutrophil-biased depletion (csf3r MO, 2 independent experiments), (J–L) of impairment of emergency granulopoiesis (nos2a MO, 2 independent experiments) and (M–O) of hepatocyte depletion (Tomm22 MO, 2 independent experiments) on CHIKV infection. (A, D, G, J and M) Disease score at 3 dpi. (B, E, H, K and N) Survival of infected zebrafish. Data pooled from the independent experiments. (C, F, I, L and O) qRT-PCR of viral E1 transcripts at 24 hpi. Mean ± s.e.m of 6 pools of 5 larvae from 2 (C, F, L) or 1 (I, O) independent experiments. (***P < 0.001; **P < 0.01; *P < 0.05; ns - not significant).