Human Cytomegalovirus Gene UL76 Induces IL-8 Expression through Activation of the DNA Damage Response
Figure 6
Requirement of UL76 for optimal IL-8 induction by HCMV.
A) HFF cells were infected with HCMV AD169 or UL76 mutant (TNUL76) at a MOI of 3. Supernatants were collected at 24 h, 48 h and 72 h post-infection and secreted IL-8 concentration was determined by ELISA. Data are expressed as means ± SD of fold induction to mock-infected cells and are representative of three similar experiments. Cells were lysed at 72 h post-infection and viral infection was confirmed by western blot (below) using an anti-IE1 antibody for the CMV viral protein and detection of tubulin as loading control.