Kaposi's Sarcoma-Associated Herpesvirus K-Rta Exhibits SUMO-Targeting Ubiquitin Ligase (STUbL) Like Activity and Is Essential for Viral Reactivation
Figure 1
Plasmids expressing E1 (Uba2/Aos), E2 (Ubc9), SUMO-2, K-Rta or empty vector (Vec) were transfected into 293T cells. SUMO, K-Rta or tubulin was probed with respective antibody. (A) K-Rta reduced total SUMO-modified protein levels in a dose-dependent manner. (B) Overexpression of SUMO but not other SUMO enzymes recover the cellular SUMO-modified proteins.