B Cells Regulate Neutrophilia during Mycobacterium tuberculosis Infection and BCG Vaccination by Modulating the Interleukin-17 Response
Figure 4
B cell deficiency results in a diminished Th1 response in BCG-immunized mice.
Wild-type or B cell-deficient mice were vaccinated with BCG and at one month post-immunization, splenic T cells were assessed for the development of Th1 response by quantification of IFN-γ-producing CD4+ T cells. A, ELISPOT analysis of the number of splenic P25-Ag85B-specific IFN-γ-producing CD4+ T cells in wild-type and μMT mice 1 month after vaccination revealed a diminished Th1 response in the B cell deficient group (** p<0.005). B, A similarly decrease in BCG-induced Th1 response was also observed in mice rendered B cell-deficient via treatment with the anti-CD20 5D2 mAb (***p<0.0005). Data shown are representative of three independent experiments with four mice per group.